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Image Search Results
Journal: Journal of the American Society of Nephrology
Article Title: Single-Cell Transcriptomic Map of the Human and Mouse Bladders
doi: 10.1681/asn.2019040335
Figure Lengend Snippet: Figure 3. ADRA2A+ ICs can be validated in human bladder tissues. (A) Violin plots showing the ADRA2A and HRH2 expression levels in each cluster. (B) IHC-P verified the ADRA2A protein expression (arrows) in human bladder tissue (Supplemental Table 2, sample ID: S004). (C) IHC-P verified the cells of the HRH2+ (arrows) in human bladder tissue (Supplemental Table 2, sample ID: S004). (D) IF analysis of the expression of VIM (red), which is an IC marker, in combination with the novel cell marker ADRA2A (green) and DNA staining by DAPI (blue) within the tissue paraffin sections from human bladder samples (Supplemental Table 2, sample ID: S004) verified these novel ICs. (E) We used another marker, HRH2 (green), to verify the same result. Scale bars, 20 mm.
Article Snippet: The slides were incubated with anti-ADRA2A antibody (rabbit anti-human/mouse, 1:1000, ab85570; Abcam), anti-TNNT1 antibody (rabbit antihuman/mouse/rat, 1:500, NBP1-32748; Novus),
Techniques: Expressing, Paraffin-embedded Immunohistochemistry, Marker, Staining